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. 1994 Feb;41(2):483-9.
doi: 10.1016/0093-691x(94)90084-v.

Fluorescent staining as a method of assessing membrane damage and post-thaw survival of ram spermatozoa

A Valcárcel  1 , M A de Las Heras, L Pérez, D F Moses, H Baldassarre
Affiliations

Fluorescent staining as a method of assessing membrane damage and post-thaw survival of ram spermatozoa

A Valcárcel et al. Theriogenology. 1994 Feb.

Abstract

We studied the relationship between motility and membrane damage, as assessed by fluorescent staining, in fresh and in frozen-thawed ram spermatozoa. Semen from Merino rams was incubated with 6-carboxyfluorescein diacetate and propidium iodide. In both fresh and frozen-thawed samples, the percentage of intact spermatozoa was lower than the motility rate, thus indicating the presence of damaged but motile spermatozoa. Freezing and thawing resulted in a marked loss of membrane integrity, whereas motility decreased to a lesser extent. There was a positive relationship (r=0.64; P<0.001) between membrane integrity immediately after thawing and motility after 8 h of incubation at 37 degrees C. These results demonstrate the usefulness of the fluorescent staining method for the prediction of ram sperm quality and post-thaw survival.

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