Essential oils as potential adulticides against two populations of Aedes aegypti, the laboratory and natural field strains, in Chiang Mai province, northern Thailand

Abstract

Essential oils derived from five plant species, celery (Apium graveolens), caraway (Carum carvi), zedoary (Curcuma zedoaria), long pepper (Piper longum), and Chinese star anise (Illicium verum), were subjected to investigation of adulticidal activity against mosquito vectors. Two populations of Aedes aegypti, the laboratory and natural field strains, collected in Chiang Mai province, northern Thailand were tested in pyrethroid-susceptibility bioassays. The results revealed that the natural field strain of A. aegypti was resistant to permethrin, with mortality rates ranging from 51 to 66%. A mild susceptibility, with mortality rates ranging from 82 to 88%, was observed in the natural field strain of A. aegypti exposed to lambdacyhalothrin, which suggested that this strain was tolerant and might be resistant to this insecticide. However, laboratory-reared A. aegypti exposed to discriminating dosages of permethrin and lambdacyhalothrin induced 100% mortality in all cases, thus indicating complete susceptibility of this strain to these insecticides. The adulticidal activity determined by topical application revealed that all five essential oils exerted a promising adulticidal efficacy against both laboratory and natural field strains of A. aegypti. Although the laboratory strain was slightly more susceptible to these essential oils than the natural field strain, no statistically significant difference was observed. Moreover, comparison of the adulticidal activity indicated that the performance of these essential oils against the two strains of A. aegypti was similar. The highest potential was established from caraway, followed by zedoary, celery, long pepper, and Chinese star anise, with an LC(50) in the laboratory strain of 5.44, 5.94, 5.96, 6.21, and 8.52 microg/mg female, respectively, and 5.54, 6.02, 6.14, 6.35, and 8.83 microg/mg female, respectively, in the field strain. These promising essential oils are, therefore, an alternative in developing and producing mosquito adulticides as an effective measure used in controlling and eradicating mosquito vectors.