In vitro down regulation of proinflammatory cytokines induced by LPS tolerance in pig CD14+ cells

Abstract

LPS tolerance is characterized by a reduced sensitivity to subsequent challenge of LPS. In human and mouse models LPS tolerance is closely associated with marked unbalanced production of leukocyte-derived inflammatory mediators which, when overexpressed, led to septic syndrome and shock. Here we characterized the in vitro induction of LPS tolerance in porcine CD14+ spleen cells in order to give insights into LPS tolerance in pigs. Following LPS stimulation, TNF-alpha and, to a minor extent, IL-8 production showed a significant reduction in CD14+ spleen monocytes that were pretreated with LPS in comparison to naïve cells, while IL-1beta production was slightly influenced by LPS stimulation and it was not affected by subsequent LPS challenge. Our findings showed that porcine CD14+ cells undergo a process, which resembles LPS tolerance, providing evidence that swine represent a valuable and useful model to perform experiments to study LPS tolerance and its biological significance.