Purine-phosphoribosyltransferase activities in rat and mouse tissues and in Ehrlich ascites-tumour cells

Abstract

1. The activities of adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase in extracts of rat and mouse liver, brain, spleen, heart and kidney, of rat bone marrow and of Ehrlich ascites-tumour cells have been measured. The specific activity of adenine phosphoribosyltransferase in tumour cells (3mmu-moles of nucleotide formed/min./mg. of protein) was between 15 and 60 times the activity of any mouse tissue examined. Hypoxanthine-phosphoribosyltransferase activity was greater in extracts of tumour cells than in mouse tissues, but the difference was not great. 2. The specific activities of both adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase in ascites-tumour cells decreased respectively by 57% and 36% 2 days after the cells had been inoculated into mice. After 4 days of tumour growth the specific activities of both enzymes increased, reaching a maximum at 10 days. 3. The activity of adenine phosphoribosyltransferase in rat-liver extracts increased steadily up to 4 days after partial hepatectomy, and was still above the control value after 14 days. Hypoxanthine-phosphoribosyltransferase activity in extracts of regenerating rat liver did not increase until the second day after operation and had begun to decrease by the fourth day. 4. From the results it was concluded that adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase may be physiologically important enzymes in rapidly growing tissues, and that inhibitors of the former enzyme have potential value in chemotherapy.