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. 1968 May;107(5):655-7.
doi: 10.1042/bj1070655.

Determination of oestrogen monoglucuronides by the Brown method

H A Blair  1 , A E Kellie
Affiliations

Determination of oestrogen monoglucuronides by the Brown method

H A Blair et al. Biochem J. 1968 May.

Abstract

In the Brown (Brown, 1955; Brown, Bulbrook & Greenwood, 1957) procedure for the determination of urinary oestrogens, losses may occur during the hydrolysis of the oestrogen conjugates and during the purification of the oestrogens thus formed. Losses during the latter stages were measured previously by adding free oestrogens to the hydrolysed urine. In the present study losses during acid and enzyme hydrolysis were measured by adding synthetic oestrogen monoglucuronides to urine.

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