Transcriptome of a Nitrosomonas europaea mutant with a disrupted nitrite reductase gene (nirK)

Abstract

Global gene expression was compared between the Nitrosomonas europaea wild type and a nitrite reductase-deficient mutant using a genomic microarray. Forty-one genes were differentially regulated between the wild type and the nirK mutant, including the nirK operon, genes for cytochrome c oxidase, and seven iron uptake genes. Relationships of differentially regulated genes to the nirK mutant phenotype are discussed.

FIG. 1.

Northern hybridizations. Intensities of hybridization to specific DNA probes for each gene were normalized between lanes by comparing signals to hybridization intensity from 23S rRNA. The bar graph on the left is a pictorial representation of levels of hybridization signal quantified from blots shown on the right. Error bars indicate standard deviations of the means from three replicate blots. NE2300 = bioB; NE0927 = pan1, the first ORF in the nirK operon; NE0924 = nirK; NE2303 = nirB. WT, wild type.