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. 2006 Jun;72(6):4450-4.
doi: 10.1128/AEM.02958-05.

Transcriptome of a Nitrosomonas europaea mutant with a disrupted nitrite reductase gene (nirK)

Catherine Mee-Hie Cho  1 , Tingfen Yan, Xueduan Liu, Liyou Wu, Jizhong Zhou, Lisa Y Stein
Affiliations

Transcriptome of a Nitrosomonas europaea mutant with a disrupted nitrite reductase gene (nirK)

Catherine Mee-Hie Cho et al. Appl Environ Microbiol. 2006 Jun.

Abstract

Global gene expression was compared between the Nitrosomonas europaea wild type and a nitrite reductase-deficient mutant using a genomic microarray. Forty-one genes were differentially regulated between the wild type and the nirK mutant, including the nirK operon, genes for cytochrome c oxidase, and seven iron uptake genes. Relationships of differentially regulated genes to the nirK mutant phenotype are discussed.

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Figures

FIG. 1.
FIG. 1.
Northern hybridizations. Intensities of hybridization to specific DNA probes for each gene were normalized between lanes by comparing signals to hybridization intensity from 23S rRNA. The bar graph on the left is a pictorial representation of levels of hybridization signal quantified from blots shown on the right. Error bars indicate standard deviations of the means from three replicate blots. NE2300 = bioB; NE0927 = pan1, the first ORF in the nirK operon; NE0924 = nirK; NE2303 = nirB. WT, wild type.
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References

    1. Beaumont, H. J. E., N. G. Hommes, L. A. Sayavedra-Soto, D. J. Arp, D. M. Arciero, A. B. Hooper, H. V. Westerhoff, and R. J. M. van Spanning. 2002. Nitrite reductase of Nitrosomonas europaea is not essential for production of gaseous nitrogen oxides and confers tolerance to nitrite. J. Bacteriol. 184:2557-2560. - PMC - PubMed
    1. Beaumont, H. J. E., S. I. Lens, W. N. M. Reijnders, H. V. Westerhoff, and R. J. M. van Spanning. 2004. Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite-sensitive transcription repressor. Mol. Microbiol. 54:148-158. - PubMed
    1. Beaumont, H. J. E., S. I. Lens, H. V. Westerhoff, and R. J. M. van Spanning. 2005. Novel nirK cluster genes in Nitrosomonas europaea are required for NirK-dependent tolerance to nitrite. J. Bacteriol. 187:6849-6851. - PMC - PubMed
    1. Beaumont, H. J. E., B. van Schooten, S. I. Lens, H. V. Westerhoff, and R. J. M. van Spanning. 2004. Nitrosomonas europaea expresses a nitric oxide reductase during nitrification. J. Bacteriol. 186:4417-4421. - PMC - PubMed
    1. Bothe, H., G. Jost, M. Schloter, B. B. Ward, and K.-P. Witzel. 2000. Molecular analysis of ammonia oxidation and denitrification in natural environments. FEMS Microbiol. Rev. 24:673-690. - PubMed

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