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. 2006 Aug;7(8):1151-4.
doi: 10.1002/cbic.200500559.

Latent blue and red fluorophores based on the trimethyl lock

Luke D Lavis  1 , Tzu-Yuan Chao, Ronald T Raines
Affiliations

Latent blue and red fluorophores based on the trimethyl lock

Luke D Lavis et al. Chembiochem. 2006 Aug.
No abstract available

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Figures

Figure 1
Figure 1
Normalized fluorescent excitation–emission spectra of pro-fluorophore 2 and AMC.
Figure 2
Figure 2
(a) Kinetic traces (λex 365 nm, λem 460 nm) and Michaelis–Menten plot (inset) for the hydrolysis of pro-fluorophore 2 (20 μM→9.8 nM) by PLE (2.5 μg/mL); kcat/KM = 2.5 × 105 M−1s−1 and KM = 8.2 μM. (b) Kinetic traces (λex 586 nm, λem 620 nm) and Michaelis–Menten plot (inset) for the hydrolysis of pro-fluorophore 4 (2.5 μM→4.9 nM) by PLE (2.5 μg/mL); apparent kcat/KM = 1.2 × 105 M−1s−1 and apparent KM = 0.14 μM.
Figure 3
Figure 3
Time course of the generation of fluorescence (λex 365 nm, λem 460 nm) from pro-fluorophore 2 (50 nM) or methylumbelliferyl acetate 3 (50 nM) in PBS containing BSA (1 mg/mL).
Figure 4
Figure 4
Unwashed mammalian cells incubated for 15 min with pro-fluorophore 4 (10 μM) at 37 °C in DMEM and counter-stained with Hoechst 33342 (5% v/v CO2(g), 100% humidity). (a) CHO K1 cells. (b) HeLa cells.
Figure 5
Figure 5
Modules in the latent fluorophores described in this work.
Scheme 1
Scheme 1
Route for the synthesis of pro-fluorophore 2, and the chemical structure of 4-methylumbelliferyl acetate (3).
Scheme 2
Scheme 2
Route for the synthesis of pro-fluorophore 4.
See this image and copyright information in PMC

References

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