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. 2006 Jun;47(3):410-5.

Mutations in Helicobacter pylori porD and oorD genes may contribute to furazolidone resistance

Zhaoliang Su  1 , Huaxi Xu, Chiyu Zhang, Shihe Shao, Liangju Li, Hua Wang, Huifang Wang, Gufeng Qiu
Affiliations
Free PMC article

Mutations in Helicobacter pylori porD and oorD genes may contribute to furazolidone resistance

Zhaoliang Su et al. Croat Med J. 2006 Jun.
Free PMC article

Abstract

Aim: To determine the rate of furazolidone resistance of Helicobacter pylori (H. pylori) isolated from gastric biopsy specimens and to explore the relationship between genetic mutations in porD and oorD genes of H. pylori and its resistance to the antibiotic.

Methods: Gastric biopsy was performed in 83 adult patients aged 31-77 years with gastric complaints. H. pylori was isolated from biopsy specimens of 46 patients. E-test and 2-fold agar dilution method were used to determine the rate of H. pylori resistance to furazolidone. The genes porD and oorD from susceptible and resistant isolates were amplified by polymerase chain reaction (PCR), and their PCR products were sequenced.

Results: Resistance to furazolidone was found in 8.7% of H. pylori isolates and 6 mutations were detected in porD and oorD genes of the resistant isolates. Three mutations--G353A, A356G, and C357T--occurred in porD and the other mutations--A041G, A122G, C349A(G)--occurred in oorD genes.

Conclusions: Changes in 6 amino acids may be associated with the resistance of H. pylori to furazolidone.

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Figures

Figure 1
Figure 1
Minimum inhibitory concentration of furazolidone for Helicobacter pylori isolates from gastric biopsy specimens obtained from 46 patients.
Figure 2
Figure 2
Agarose gel electropherogram of porD (A) and oorD (B). A. Markers (from the left): NCTC11637, ZJ0021, ZJ0023, ZJ0027, ZJ0024, ZJ0021, and negative control. B. Markers (from the left): NCTC11637, ZJ0021, ZJ0024, ZJ0003, and negative control.
Figure 3
Figure 3
Analysis of porD mutations from furazolidone-resistant strains of Helicobacter pylori showed that 353 glutamate was replaced by aspartate, 356 alanine by threonine, and 357 threonine by valine.
Figure 4
Figure 4
Analysis of oorD mutations from furazolidone-resistant strains of Helicobacter pylori showed that 041 threonine was replaced by alanine, 122 isoleucine by valine, and 349 asparagine by lysine.
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