[Development of ELISA-kit of quantitative analysis for Zearalenone]

Abstract

Objective: To develope a rapid, sensitive, quantitative ELISA-kit for Zearalenone and determine zearalenone in cereals.

Methods: On the base of monoclonal antibodies against ZEN, apply indirect ELISA to study the performance parameter of the kit.

Results: The limited concentration of detection of the ELISA-kit was 1ng/ml, linear range was 1-200 ng/ml, the linear equation was Y = 0.99 - 0.40 x (R2 = 0.99). The inhibition concentration of 50% against ZEN was 16.3 ng/ml. The average recovery rate of spiked corn and wheat was 96.5% and 95.5%, respectively, the coefficient of variant was 13.2% and 10.9%, respectively. The kit can be stored at 4 degrees C over 6 months. The cross reaction rate with the other mycotoxins was less than 1%, and coefficient of variant within-laboratory and between-laboratory was less than 15% and less than 20%, respectively. Detecting the VICAM sample with ELISA method and HPLC method, the results were within the range of the sample, and there was no statistic difference between the two methods.

Conclusion: This ELISA-kit was quick, sensitive, stable and specific and can be used to determine ZEN in cereals.