Interaction of the onset of spring and elevated atmospheric CO2 on ragweed (Ambrosia artemisiifolia L.) pollen production

Abstract

Increasing atmospheric carbon dioxide is responsible for climate changes that are having widespread effects on biological systems. One of the clearest changes is earlier onset of spring and lengthening of the growing season. We designed the present study to examine the interactive effects of timing of dormancy release of seeds with low and high atmospheric CO2 on biomass, reproduction, and phenology in ragweed plants (Ambrosia artemisiifolia L.), which produce highly allergenic pollen. We released ragweed seeds from dormancy at three 15-day intervals and grew plants in climate-controlled glass-houses at either ambient or 700-ppm CO2 concentrations, placing open-top bags over influorescences to capture pollen. Measurements of plant height and weight; inflorescence number, weight, and length; and days to anthesis and anthesis date were made on each plant, and whole-plant pollen productivity was estimated from an allometric-based model. Timing and CO2 interacted to influence pollen production. At ambient CO2 levels, the earlier cohort acquired a greater biomass, a higher average weight per inflorescence, and a larger number of influorescences; flowered earlier; and had 54.8% greater pollen production than did the latest cohort. At high CO2 levels, plants showed greater biomass and reproductive effort compared with those in ambient CO2 but only for later cohorts. In the early cohort, pollen production was similar under ambient and high CO2, but in the middle and late cohorts, high CO2 increased pollen production by 32% and 55%, respectively, compared with ambient CO2 levels. Overall, ragweed pollen production can be expected to increase significantly under predicted future climate conditions.

Figure 1

End-of-season biomass measures of A. artemisiifolia: aboveground biomass (A) and plant height (B) for three springtime dormancy release cohorts at two CO₂ concentrations (380 ppm and 700 ppm). Error bars indicate 95% confidence intervals.

Figure 2

End-of-season reproductive measures of A. artemisiifolia: number of inflorescences (A) and inflorescence weight (B) for three springtime dormancy release cohorts at two CO₂ concentrations (380 ppm and 700 ppm). Error bars indicate 95% confidence intervals.

Figure 3

Phenology measures of A. artemisiifolia: number of days to anthesis (A) and anthesis date (B) for three springtime dormancy release cohorts at two CO₂ concentrations (380 ppm and 700 ppm). Error bars indicate 95% confidence intervals.

Figure 4

Pollen production in A. artemisiifolia for three springtime dormancy release cohorts grown at two CO₂ concentrations (380 ppm and 700 ppm). Error bars indicate 95% confidence intervals.