[Long-term storage of obligate anaerobic microorganisms in glycerol]
- PMID: 16761574
[Long-term storage of obligate anaerobic microorganisms in glycerol]
Abstract
We evaluated the possibility of storing the cultures of obligate anaerobic microorganisms (clostridia. acetogenic and sulfate-reducing bacteria, and methanogenic archaea) in 25% glycerol at -70 degrees C for a long time (up to 3 years). This method of storage is adequate to preserve cell viability in most obligate anaerobes.