doi: 10.1111/j.1744-313x.1991.tb00007.x.
PCR-SSO typing for HLA-DRB alleles
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- PMID: 1676912
- DOI: 10.1111/j.1744-313x.1991.tb00007.x
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PCR-SSO typing for HLA-DRB alleles
Eur J Immunogenet.
1991 Feb-Apr.
Abstract
Polymerase chain reaction (PCR) amplification of the second exon of DRB genes from genomic DNA is described. Sequence-specific oligonucleotide (SSO) probes have been designed to allow for the typing of DRB alleles. The methods are described in detail and the results of a trial on both homozygous typing cells and restriction fragment length polymorphism (RFLP)-typed local caucasoid controls are given. The ease of use of this form of DRB typing is emphasized and potential complications are discussed.
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