Stimulation of energy metabolism by alpha-adrenergic agonists in primary cultures of astrocytes

Abstract

Noradrenaline effects on glucose oxidation were studied in primary cultures of astrocytes. CO2 formation from labeled glucose was enhanced in the presence of noradrenaline. The stimulatory effect by noradrenaline was exerted both on lactate formation (approximately 20%) and on tricarboxylic acid activity (CO2 production from glutamate) (approximately 40%). The effect was, at least partly, exerted on the alpha-ketoglutarate dehydrogenase step. The EC50 value for noradrenaline on lactate formation was significantly lower (60 nM) than that on oxidative metabolism (1,900 nM). Studies with specific adrenergic agonists and antagonists showed that various receptor subtypes are involved. Thus, the effect on lactate formation was mediated exclusively by stimulation of an alpha 1 receptor whereas oxidative metabolism was enhanced by both alpha 1 and alpha 2 receptor stimulation. No effects were exerted by beta receptor agonists or antagonists.