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Comment
. 2006 Sep 1;91(5):2009-10, discussion 2011-2.
doi: 10.1529/biophysj.106.085126. Epub 2006 Jun 16.

Fingerprinting DHFR in single-molecule AFM studies

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Comment

Fingerprinting DHFR in single-molecule AFM studies

Sri Rama Koti Ainavarapu et al. Biophys J. .
No abstract available

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Figures

FIGURE 1
FIGURE 1
Force-extension curves obtained using the chimeric polyprotein (I27-DHFR)4 in the absence (A) and in the presence (C) of 190 μM MTX. The corresponding histograms of unfolding forces of DHFR are shown in B and D, respectively.

Comment on

References

    1. Ainavarapu, S. R., L. Li, C. L. Badilla, and J. M. Fernandez. 2005. Ligand binding modulates the mechanical stability of dihydrofolate reductase. Biophys. J. 89:3337–3344. - PMC - PubMed
    1. Junker, J. P., K. Hell, M. Schlierf, W. Neupert, and M. Rief. 2005. Influence of substrate binding on the mechanical stability of mouse dihydrofolate reductase. Biophys. J. 89:L46–L48. - PMC - PubMed
    1. Carrion-Vazquez, M., P. E. Marszalek, A. F. Oberhauser, and J. M. Fernandez. 1999. Atomic force microscopy captures length phenotypes in single proteins. Proc. Natl. Acad. Sci. USA. 96:11288–11292. - PMC - PubMed
    1. Carrion-Vazquez, M., A. F. Oberhauser, S. B. Fowler, P. E. Marszalek, S. E. Broedel, J. Clarke, and J. M. Fernandez. 1999. Mechanical and chemical unfolding of a single protein: a comparison. Proc. Natl. Acad. Sci. USA. 96:3694–3699. - PMC - PubMed
    1. Li, H., A. F. Oberhauser, S. B. Fowler, J. Clarke, and J. M. Fernandez. 2000. Atomic force microscopy reveals the mechanical design of a modular protein. Proc. Natl. Acad. Sci. USA. 97:6527–6531. - PMC - PubMed

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