doi: 10.1128/JVI.15.5.1057-1064.1975.
Blocked and unblocked 5' termini in reovirus genome RNA
Affiliations
- PMID: 16789145
- PMCID: PMC354559
- DOI: 10.1128/JVI.15.5.1057-1064.1975
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Blocked and unblocked 5' termini in reovirus genome RNA
J Virol.
1975 May.
Abstract
Uniformly (32)P-labeled, double-stranded genome RNA isolated from purified reovirus contains two types of 5'-terminal sequences. One strand contains a phosphatase-resistant 5'-terminal structure, XpppG(*)pCpU, which is also present in the viral mRNA. The 5' blocking group, X, is removed by beta-elimination indicating that it is a nucleoside containing free 2',3'-hydroxyls. G(*)pC is an alkaline-resistant, 2'-O-methylated sequence. The other strand contains a phosphatase-sensitive 5' sequence, ppGpPupPyp. The results are discussed in relation to blocked 5'-terminal structures in other viral and cellular RNAs.
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