Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006:409:39-52.
doi: 10.1016/S0076-6879(05)09003-8.

Human DNA ligases I, III, and IV-purification and new specific assays for these enzymes

Xi Chen  1 , John Pascal, Sangeetha Vijayakumar, Gerald M Wilson, Tom Ellenberger, Alan E Tomkinson
Affiliations

Human DNA ligases I, III, and IV-purification and new specific assays for these enzymes

Xi Chen et al. Methods Enzymol. 2006.

Abstract

The joining of DNA strand breaks by DNA ligases is required to seal Okazaki fragments during DNA replication and to complete almost all DNA repair pathways. In human cells, there are multiple species of DNA ligase encoded by the LIG1, LIG3, and LIG4 genes. Here we describe protocols to overexpress and purify recombinant DNA ligase I, DNA ligase IIIbeta, and DNA ligase IV/XRCC4 and the assays used to purify and distinguish between these enzymes. In addition, we describe a fluorescence-based ligation assay that can be used for high throughput screening of chemical libraries.

PubMed Disclaimer

Publication types

LinkOut - more resources