Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Jun;13(6):575-85.
doi: 10.1016/j.chembiol.2006.03.008.

Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis

Xin-Ying Jia  1 , Zhen-Hua Tian, Lei Shao, Xu-Dong Qu, Qun-Fei Zhao, Jian Tang, Gong-Li Tang, Wen Liu
Affiliations

Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis

Xin-Ying Jia et al. Chem Biol. 2006 Jun.

Abstract

The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5-chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit.

PubMed Disclaimer

Publication types

MeSH terms

Associated data

LinkOut - more resources