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. 2006 Apr;12(2):146-52.
doi: 10.1080/13550280600748946.

Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

Melissa J Churchill  1 , Paul R Gorry, Daniel Cowley, Luxshimi Lal, Secondo Sonza, Damian F J Purcell, Katherine A Thompson, Dana Gabuzda, Justin C McArthur, Carlos A Pardo, Steven L Wesselingh
Affiliations

Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

Melissa J Churchill et al. J Neurovirol. 2006 Apr.

Abstract

The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.

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References

    1. Curr HIV/AIDS Rep. 2005 Jun;2(2):90-7 - PubMed
    1. Nat Rev Immunol. 2005 Jan;5(1):69-81 - PubMed
    1. Ann Neurol. 1996 Jun;39(6):705-11 - PubMed
    1. AIDS. 1999 Jan 14;13(1):1-22 - PubMed
    1. Ann Neurol. 2001 Jun;49(6):745-52 - PubMed

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