Development of restricted-access media supports and their application to the direct analysis of biological fluid samples via high-performance liquid chromatography
- PMID: 16800052
- DOI: 10.1007/s00216-005-0253-9
Development of restricted-access media supports and their application to the direct analysis of biological fluid samples via high-performance liquid chromatography
Erratum in
- Anal Bioanal Chem. 2006 Aug;385(8):1580
Abstract
A quick overview of published methods for analyzing compounds in complex biological samples reveals that the most difficult step is the clean-up or extraction of a required compound from the matrix. The strategy required to analyze exogenous compounds in biological fluids depends greatly upon the nature of the compound and upon the biomatrix. Coupled-column separation using restricted-access media as the first dimension in order to exclude macromolecules and retain micromolecules has been successfully used for a number of biological fluids. This paper presents the history of the development of restricted-access media supports and of their application to the direct injection of biological fluid samples in high-performance liquid chromatography.
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