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. 2006 Dec;132(12):811-6.
doi: 10.1007/s00432-006-0130-8. Epub 2006 Jun 28.

DeltaNp63 protein expression in uterine cervical and endometrial cancers

Zhenhua Lin  1 , Mingzhu Liu, Zhuhu Li, Changheon Kim, Eungseok Lee, Insun Kim
Affiliations

DeltaNp63 protein expression in uterine cervical and endometrial cancers

Zhenhua Lin et al. J Cancer Res Clin Oncol. 2006 Dec.

Abstract

Purpose: To investigate the significance of p63 expression in uterine cervical and endometrial cancers.

Materials and methods: DeltaNp63 protein expression was studied in a variety of 127 cases of uterine cervical lesions (20 non-neoplastic cervices, 43 cervical intraepithelial neoplasia [CIN], 54 squamous cell carcinomas (SCCs), 40 adenocarcinomas, and 13 other histologic types) and 30 endometrioid type of endometrial adenocarcinomas by using immunohistochemistry. One SCC cell line (ME-180) and one adenocarcinoma cell line (HeLa) were also included.

Results: In uterine cervix, the expression of DeltaNp63 was increased with progression of CIN, and positive in all SCCs, transitional cell carcinomas, and adenoid basal carcinoma, but negative in all adenocarcinomas. Adenosquamous cell carcinoma and mixed neuroendocrine and squamous cell carcinoma were positive in squamous component, but not in adenocarcinoma and neuroendocrine carcinoma components. ME-180 cell line was positive, whereas HeLa cell line was negative. Endometrioid type of endometrial adenocarcinomas showed a positive staining in glandular (26.7%) and squamous component.

Conclusions: Immunohistochemical staining for DeltaNp63 is a powerful marker for squamous differentiation and useful in exclusion of glandular and neuroendocrine differentiation in uterine cervical cancers, but not always in endometrial cancers.

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Figures

Fig. 1
Fig. 1
Immunostaining for ΔNp63. a ΔNp63-positive cells are seen in basal and suprabasal cells of the squamous epithelium of uterine cervix. b ΔNp63-positive cells beneath the endocervical glands represent reserve cells. c The cells in the lower one-third of the squamous epithelium of uterine cervix in mild dysplasia are ΔNp63-positive. d Squamous epithelial cells of almost full thickness in CIN3 are positive for ΔNp63. e The cells in keratinizing squamous cell carcinoma of the uterine cervix are ΔNp63-positive. The central keratinizing cells are ΔNp63-negative. f Most of the cells in nonkeratinizing squamous cell carcinoma are ΔNp63-positive
Fig. 2
Fig. 2
a Adenocarcinoma of cervix is entirely negative for ΔNp63. b The mixed neuroendocrine carcinoma of cervix. Only squamous component is ΔNp63-positive. c Grade 1 adenocarcinoma of endometrium is entirely negative for ΔNp63. d Grade 2 adenocarcinoma of endometrium shows some ΔNp63-positive cells. e ME-180 cell line shows strong nuclear staining for ΔNp63. f HeLa cell line is entirely negative for ΔNp63
See this image and copyright information in PMC

References

    1. Barbareschi M, Pecciarini L, Cangi MG (2001) P63, a p53 homologue, is a selective nuclear marker of myoepithelial cells of the human breast. Am J Surg Pathol 25:1054–1060 - DOI - PubMed
    1. Benard J, Douc-Rasy S, Ahomadegbe (2003) TP53 family members and human cancers. Hum Mutat 21:182–191 - DOI - PubMed
    1. Burstein DE, Nagi C, Wang BY, Unger P (2004) Immunohistochemical detection of p53 homolog p63 in solid cell nests, papillary thyroid carcinoma, and Hashimoto’s thyroiditis: a stem cell hypothesis of papillary carcinoma oncogenesis. Hum Pathol 35:465–473 - DOI - PubMed
    1. Chen YK, Hsue SS, Lin LM (2004) Expression of p63 (TA and ΔN isoforms) in human primary well differentiated buccal carcinomas. Int J Oral Maxillolfacial Surg 33:493–497 - DOI - PubMed
    1. Di Como CJ, Urist MJ, Babayan I, Drobnjak M, Hedvat CV, Teruya-Feldstein J, Pohar K, Hoos A, Cordon-Cardo C (2002) p63 expression profiles in human normal and tumor tissues. Clin Cancer Res 8:494–501 - PubMed

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