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. 2006 Jul;72(7):5086-8.
doi: 10.1128/AEM.00157-06.

In vivo transduction of an Stx-encoding phage in ruminants

Nancy A Cornick  1 , Amy F Helgerson, Volker Mai, Jennifer M Ritchie, David W K Acheson
Affiliations

In vivo transduction of an Stx-encoding phage in ruminants

Nancy A Cornick et al. Appl Environ Microbiol. 2006 Jul.

Abstract

We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.

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Figures

FIG. 1.
FIG. 1.
Geometric mean of fecal shedding of donor E. coli O157:H7 (1:361Δstx2:kan) (•) and recipient E. coli (C43) (○) strains from (A) control sheep, (B) sheep given 0.125 mg/kg/day of enrofloxacin, and (C) sheep given 0.25 mg/kg/day of enrofloxacin. Enrofloxacin was given on days 3 to 5 p.i. (samples at days 4 to 6 p.i. are shown in the bracketed areas). Bars indicate the standard errors.
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