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. 2006 Jul;44(7):2673-5.
doi: 10.1128/JCM.02514-05.

Molecular typing reveals a unique clone of Salmonella enterica serotype typhi among Indian strains

Dinesh S Chandel, Rama Chaudhry, Aparajit B Dey, Pawan Malhotra

Molecular typing reveals a unique clone of Salmonella enterica serotype typhi among Indian strains

Dinesh S Chandel et al. J Clin Microbiol. 2006 Jul.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Chemiluminescence X-ray signals generated by IS200 probing. Lanes 2 to 7, the unique IST-II pattern shared by six strains with two IS200-probed DNA fragments; lanes 1 and 8 to 16, the IST-I pattern shows a serotype Typhi-specific pattern with 11 to 14 apparent copies. Lanes A and B, known serovars Paratyphi A (A) and Paratyphi B (B) with six and five probed fragments, respectively, are included as controls. Notably, the two hybridized DNA fragments shown by the arrows are consistent in all serotype Typhi strains. M, molecular size standards.
FIG. 2.
FIG. 2.
Comparative cluster analysis of IS200 and PFGE profiles. (A) The unique IST-II pattern (strains A2 to A7) revealed reduced apparent IS200 copies. The IST-I cluster shows a serotype Typhi-specific predominant pattern and further subtypes Ia to Ic. Serotype Paratyphi A (SPA) and serotype Paratyphi-B (SPB), included as controls, form a separate cluster with distinct band patterns. (B) PFGE also identified strains included in IST-II as a separate clone, PFP-II. Large genome alterations were revealed in PFP-II, the unique cluster.
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