Effects of inositol hexaphosphate on proliferation of HT-29 human colon carcinoma cell line

Abstract

Aim: To investigate the effects of inositol hexaphosphate (IP(6)) on proliferation of HT-29 human colon carcinoma cell line.

Methods: Cells were exposed to various concentrations (0, 1.8, 3.3, 5.0, 8.0, 13.0 mmol/L) of IP(6) for a certain period of time. Its effect on growth of HT-29 cells was measured by MTT assay. The expressions of cell cycle regulators treated with IP(6) for 2 d were detected by immunocytochemistry.

Results: IP(6) inhibited the HT-29 cell growth in a dose- and time-dependent manner. Analysis of cell cycle regulator expression revealed that IP(6) reduced the abnormal expression of P53 and PCNA and induced the expression of P21.

Conclusion: IP(6) has potent inhibitory effect on proliferation of HT-29 cells by modulating the expression of special cell cycle regulators.

Figure 1

MTT assay showing the effect of IP₆ on the growth of HT-29 cells after treated for 0 (A), 6 (B), 12 (C), 24 h (D).

Figure 2

Expression of p53 (A, B), p21 (C, D) and PCNA (E, F) in untreated control and IP₆-treated cells. The slides were counterstained with hematoxylin (× 400, × 100,× 400, respectively).