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. 2006 Oct;33(10):1214-7.
doi: 10.1007/s00259-006-0178-3. Epub 2006 Jul 11.

In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals

F Forrer  1 , R Valkema, B Bernard, N U Schramm, J W Hoppin, E Rolleman, E P Krenning, M de Jong
Affiliations

In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals

F Forrer et al. Eur J Nucl Med Mol Imaging. 2006 Oct.

Abstract

Purpose: In vivo quantification of radiopharmaceuticals has great potential as a tool in developing new drugs. We investigated the accuracy of in vivo quantification with multi-pinhole single-photon emission computed tomography (SPECT) in rats.

Methods: Fifteen male Lewis rats with different stages of renal dysfunction were injected with 50 MBq 99mTc-dimercaptosuccinic acid. Four to six hours after injection, SPECT of the kidneys was acquired with a new four-headed multi-pinhole collimator camera. Immediately after imaging the rats were sacrificed and the kidneys were counted in a gamma-counter to determine the absorbed activity. SPECT data were reconstructed iteratively and regions of interest (ROIs) were drawn manually. The absolute activity in the ROIs was determined.

Results: Uptake values ranging from 0.71% to 21.87% of the injected activity were measured. A very strong linear correlation was found between the determined activity in vivo and ex vivo (r2=0.946; slope m=1.059).

Conclusion: Quantification in vivo using this multi-pinhole SPECT system is highly accurate.

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