Tickborne encephalitis virus, Norway and Denmark

Abstract

Serum from 2 Norwegians with tickborne encephalitis (TBE) (1 of whom was infected in Denmark) and 810 Norwegian ticks were tested for TBE virus (TBEV) RNA by reverse transcription-polymerase chain reaction. Sequencing and phylogenetic analysis were performed. This is the first genome detection of TBEV in serum from Norwegian patients.

Figure

Consensus phylogenetic tree (Fitch-Margoliash) based on the E protein encoding sequence (nucleotides 1323–1765). Only bootstrap support values >70% are shown. Newly identified tickborne encephalitis virus (TBEV) strains from Norway and Denmark are underlined. Abbreviations and accession numbers for viruses and TBEV strains: Powassan virus, POWV, strain LB (L06436); Langat virus, LGTV, strain TP21 (AF253419); Kyasanur forest disease virus, KFDV, (X74111.1); Omsk hemorrhagic fever virus, OHFV, (X66694.1); TBEV, strain IR991M4 (AB049349); TBEV, strain IR992M3 (AB049350); TBEV, strain IR992M7 (AB049351); TBEV, strain IR992F7 (AB049352); TBEV, strain IR992F13 (AB049353); TBEV, strain IR991M1 (AB049348); TBEV, strain Aina (AF091006); TBEV, strain Vasilchenko (AF069066); TBEV, strain Latvia 1–96 (AJ415565); TBEV, strain Oshima (AB022292); TBEV, strain Sofjin (X03870.1); TBEV, strain KH98–10 (AB022297); TBEV, strain Tblood (AF091019); TBEV, strain RK1424 (AF091016); TBEV, strain N132 (AF091013); TBEV, strain 263 (U27491); TBEV, strain Latvia-12718–00 (AJ319586); TBEV, strain Hypr (U39292); TBEV, strain Neudoerfl (U27495); TBEV, strain Latvia-9793–00 (AJ319585); TBEV, strain Latvia-8110–00 (AJ319583); TBEV, strain Kumlinge-A52 (X60286); TBEV, strain Latvia-8369–00 (AJ19584); TBEV, strain Latvia-11686–00 (AJ319582); TBEV, strain Lithuania-1–00 (AJ414703); louping Ill virus (LIV), strain UK369/T2 (Y07863.1); LIV, strain Turkey (LO1265); LIV, strain Greece (X77732); LIV, strain Spain (X77470.1); LIV, strain Japan 3248/49/P10 (M94956.1).