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. 2006 Jul;12(7):1145-8.
doi: 10.3201/eid1207.051555.

Replicon typing of plasmids encoding resistance to newer beta-lactams

Alessandra Carattoli  1 , Vivi Miriagou, Alessia Bertini, Alexandra Loli, Celine Colinon, Laura Villa, Jean M Whichard, Gian Maria Rossolini
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Replicon typing of plasmids encoding resistance to newer beta-lactams

Alessandra Carattoli et al. Emerg Infect Dis. 2006 Jul.

Abstract

Polymerase chain reaction-based replicon typing represents a novel method to describe the dissemination and follow the evolution of resistance plasmids. We used this approach to study 26 epidemiologically unrelated Enterobacteriaceae and demonstrate the dominance of incompatibility (Inc) A/C or Inc N-related plasmids carrying some emerging resistance determinants to extended-spectrum cephalosporins and carbapenems.

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Figures

Figure
Figure
Restriction enzyme analysis of plasmids analyzed in this study. Numbers and letters above each lane indicate strain reference names as defined in the Table. M is the1-kb Plus DNA ladder (Gibco-BRL, Gaithersburg, MD, USA). Plasmids were transferred by conjugation or transformation in Escherichia coli K12 strains and purified by Midi-prep purification kit. Plasmids were digested with restriction endonuclease PstI and separated by agarose (1%) gel electrophoresis in 1× TBE buffer. DNA was stained with ethidium bromide and visualized under UV light. The replicons and the β-lactamase genes carried by each group of plasmids are indicated below the figure.
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