Human cytomegalovirus. III. Virus-induced DNA polymerase
- PMID: 168404
- PMCID: PMC354668
- DOI: 10.1128/JVI.16.2.298-310.1975
Human cytomegalovirus. III. Virus-induced DNA polymerase
Abstract
Infection of WI-38 human fibroblasts with human cytomegalovirus (CMV) led to the stimulation of host cell DNA polymerase synthesis and induction of a novel virus-specific DNA polymerase. This cytomegalovirus-induced DNA polymerase was purified and separated from host cell enzymes by DEAE-cellulose and phosphocellulose column chromatographies. It can be distinguished from host cell enzymes by chromatographic behavior, template primer specificity, sedimentation property, and the requirement of salt for maximal activity. This virus-induced enzyme has a sedimentation coefficient of 9.2S and is found in both the nuclei and cytoplasm of virus-infected cells, but not in uninfected cells. This enzyme could efficiently use activated calf-thymus DNA, oly(dA)-oligo(dT)12-18, and poly(dC)-oligo(dG)12-18 as template primers, especially poly(dA)-oligo(dT)12-18, but it could not use poly(rA)-oligo(dT)12-18, poly(rC)-oligo(dG)12-18, or oligo(dT)12-18. The enzyme requires Mg2+ for maximal activity, is sensitive to p-hydroxymercuribenzoate, and is not a zinc metalloenzyme. In addition, the cytomegalovirus-induced DNA polymerase activity can be enhanced by adding 0.06 to 0.12 M NaCl or 0.03 to 0.06 M (NH4)2SO4 to the reaction mixture.
Similar articles
-
Purification and characterization of varicella-zoster virus-induced DNA polymerase.J Virol. 1978 May;26(2):249-56. doi: 10.1128/JVI.26.2.249-256.1978. J Virol. 1978. PMID: 207886 Free PMC article.
-
Marek's disease herpesvirus-induced DNA polymerase.J Virol. 1974 Nov;14(5):1209-19. doi: 10.1128/JVI.14.5.1209-1219.1974. J Virol. 1974. PMID: 4473569 Free PMC article.
-
Characterization of DNA polymerase associated with nuclear membrane fractions from uninfected and adenovirus 2-infected KB cells.Biochim Biophys Acta. 1976 Oct 18;447(3):340-52. doi: 10.1016/0005-2787(76)90057-5. Biochim Biophys Acta. 1976. PMID: 974129
-
Comparative study of herpes group virus-induced DNA polymerases.Intervirology. 1979;12(2):73-83. doi: 10.1159/000149071. Intervirology. 1979. PMID: 231586
-
Isolation of DNA polymerase gamma from an adenovirus 2 DNA replication complex.J Virol. 1975 Jun;15(6):1507-10. doi: 10.1128/JVI.15.6.1507-1510.1975. J Virol. 1975. PMID: 1142477 Free PMC article.
Cited by
-
Pyrrolo[2,3-d]pyrimidine nucleosides as inhibitors of human cytomegalovirus.Antimicrob Agents Chemother. 1987 Apr;31(4):544-50. doi: 10.1128/AAC.31.4.544. Antimicrob Agents Chemother. 1987. PMID: 3037998 Free PMC article.
-
Preservation of natural endothelial cytopathogenicity of cytomegalovirus by propagation in endothelial cells.Arch Virol. 1991;117(3-4):143-64. doi: 10.1007/BF01310761. Arch Virol. 1991. PMID: 1850227
-
Sequence and transcription analysis of the human cytomegalovirus DNA polymerase gene.J Virol. 1987 Jan;61(1):125-33. doi: 10.1128/JVI.61.1.125-133.1987. J Virol. 1987. PMID: 3023690 Free PMC article.
-
2'-fluoro-5-iodo-aracytosine, a potent and selective anti-herpesvirus agent.Antimicrob Agents Chemother. 1980 May;17(5):803-6. doi: 10.1128/AAC.17.5.803. Antimicrob Agents Chemother. 1980. PMID: 6249196 Free PMC article.
-
Stimulation of cellular thymidine kinases by human cytomegalovirus.J Virol. 1977 Oct;24(1):13-21. doi: 10.1128/JVI.24.1.13-21.1977. J Virol. 1977. PMID: 20515 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources