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Review
. 2005;51(1):21-2.

Recommended method for recovery of Toxocara and other geohelminth eggs from soil

Affiliations
  • PMID: 16841685
Review

Recommended method for recovery of Toxocara and other geohelminth eggs from soil

Hanna Mizgajska-Wiktor. Wiad Parazytol. 2005.

Abstract

The flotation method elaborated for recovery of Toxocara and other geohelminth eggs from soil is described. Soil samples of about 500 ml volume are picked from 3-cm superficial layer of the ground. In the laboratory, 40 g of dry and sifted material is analysed according to following procedure: 1 h standing, 20 minutes shaking and 3 minutes centrifugation (1500 rpm) in 5% sodium hydroxide (NaOH), then centrifugation, like above, with H2O for washing the sample and next with the saturated sodium nitrate (NaNO3) for flotation the eggs. Specimen is prepared by placing a cover slip on the positive meniscus of the flotation liquid.

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