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. 2006 Aug;12(8):809-12.
doi: 10.1111/j.1469-0691.2006.01452.x.

Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

H Mayta  1 , M Calderon, J Taverna, S Montenegro, J Balqui, K Campos, I Tuero, J Arevalo, A Vivar, R H Gilman
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Free article

Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients

H Mayta et al. Clin Microbiol Infect. 2006 Aug.
Free article

Abstract

Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.

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