Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Nov;129(3):833-44.
doi: 10.1093/genetics/129.3.833.

Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome

B I Osborne  1 , C A Corr, J P Prince, R Hehl, S D Tanksley, S McCormick, B Baker
Affiliations

Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome

B I Osborne et al. Genetics. 1991 Nov.

Abstract

We have investigated the distribution of transposed Acs in the tomato genome. Our approach has been to clone the regions flanking the T-DNAs and transposed Acs from two transgenic lines of tomato and place these sequences on the tomato restriction fragment length polymorphism (RFLP) map. The distribution of transposed Acs around the T-DNA and at locations unlinked to the T-DNA indicates that Ac transposes to linked and unlinked sites in tomato as it does in maize. The structure and terminal sequence of these cloned elements shows that Ac remains intact after transposition. We discuss these results and their bearing on gene tagging strategies using Ac and Ds.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844-8 - PubMed
    1. Nature. 1984 Jan 12-18;307(5947):127-30 - PubMed
    1. EMBO J. 1987 Jun;6(6):1547-54 - PubMed
    1. Genetics. 1984 Oct;108(2):471-85 - PubMed
    1. Science. 1984 Mar 23;223(4642):1265-8 - PubMed

Publication types

Substances