Two glucose-sensing pathways converge on Rgt1 to regulate expression of glucose transporter genes in Saccharomyces cerevisiae
- PMID: 16844691
- DOI: 10.1074/jbc.M603636200
Two glucose-sensing pathways converge on Rgt1 to regulate expression of glucose transporter genes in Saccharomyces cerevisiae
Abstract
The yeast Saccharomyces cerevisiae deploys two different types of glucose sensors on its cell surface that operate in distinct glucose signaling pathways: the glucose transporter-like Snf3 and Rgt2 proteins and the Gpr1 receptor that is coupled to Gpa2, a G-protein alpha subunit. The ultimate target of the Snf3/Rgt2 pathway is Rgt1, a transcription factor that regulates expression of HXT genes encoding glucose transporters. We have found that the cAMP-dependent protein kinase A (PKA), which is activated by the Gpr1/Gpa2 glucose-sensing pathway and by a glucose-sensing pathway that works through Ras1 and Ras2, catalyzes phosphorylation of Rgt1 and regulates its function. Rgt1 is phosphorylated in vitro by all three isoforms of PKA, and this requires several serine residues located in PKA consensus sequences within Rgt1. PKA and the consensus serine residues of Rgt1 are required for glucose-induced removal of Rgt1 from the HXT promoters and for induction of HXT expression. Conversely, overexpression of the TPK genes led to constitutive expression of the HXT genes. The PKA consensus phosphorylation sites of Rgt1 are required for an intramolecular interaction that is thought to regulate its DNA binding activity. Thus, two different glucose signal transduction pathways converge on Rgt1 to regulate expression of glucose transporters.
Similar articles
-
How the Rgt1 transcription factor of Saccharomyces cerevisiae is regulated by glucose.Genetics. 2005 Feb;169(2):583-94. doi: 10.1534/genetics.104.034512. Epub 2004 Oct 16. Genetics. 2005. PMID: 15489524 Free PMC article.
-
Integration of transcriptional and posttranslational regulation in a glucose signal transduction pathway in Saccharomyces cerevisiae.Eukaryot Cell. 2006 Jan;5(1):167-73. doi: 10.1128/EC.5.1.167-173.2006. Eukaryot Cell. 2006. PMID: 16400179 Free PMC article.
-
The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae.Biochim Biophys Acta. 2015 Jul;1850(7):1362-7. doi: 10.1016/j.bbagen.2015.03.006. Epub 2015 Mar 22. Biochim Biophys Acta. 2015. PMID: 25810078
-
Function and regulation of yeast hexose transporters.Microbiol Mol Biol Rev. 1999 Sep;63(3):554-69. doi: 10.1128/MMBR.63.3.554-569.1999. Microbiol Mol Biol Rev. 1999. PMID: 10477308 Free PMC article. Review.
-
Novel sensing mechanisms and targets for the cAMP-protein kinase A pathway in the yeast Saccharomyces cerevisiae.Mol Microbiol. 1999 Sep;33(5):904-18. doi: 10.1046/j.1365-2958.1999.01538.x. Mol Microbiol. 1999. PMID: 10476026 Review.
Cited by
-
D-Xylose Sensing in Saccharomyces cerevisiae: Insights from D-Glucose Signaling and Native D-Xylose Utilizers.Int J Mol Sci. 2021 Nov 17;22(22):12410. doi: 10.3390/ijms222212410. Int J Mol Sci. 2021. PMID: 34830296 Free PMC article. Review.
-
Regulation and metabolic engineering strategies for permeases of Saccharomyces cerevisiae.World J Microbiol Biotechnol. 2019 Jul 8;35(7):112. doi: 10.1007/s11274-019-2684-z. World J Microbiol Biotechnol. 2019. PMID: 31286266 Review.
-
Reconstruction and logical modeling of glucose repression signaling pathways in Saccharomyces cerevisiae.BMC Syst Biol. 2009 Jan 14;3:7. doi: 10.1186/1752-0509-3-7. BMC Syst Biol. 2009. PMID: 19144179 Free PMC article.
-
The glucose metabolite methylglyoxal inhibits expression of the glucose transporter genes by inactivating the cell surface glucose sensors Rgt2 and Snf3 in yeast.Mol Biol Cell. 2016 Mar 1;27(5):862-71. doi: 10.1091/mbc.E15-11-0789. Epub 2016 Jan 13. Mol Biol Cell. 2016. PMID: 26764094 Free PMC article.
-
SNF3 as High Affinity Glucose Sensor and Its Function in Supporting the Viability of Candida glabrata under Glucose-Limited Environment.Front Microbiol. 2015 Dec 1;6:1334. doi: 10.3389/fmicb.2015.01334. eCollection 2015. Front Microbiol. 2015. PMID: 26648919 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases