ASGS: an alternative splicing graph web service

Abstract

Alternative transcript diversity manifests itself a prime cause of complexity in higher eukaryotes. The Alternative Splicing Graph Server (ASGS) is a web service facilitating the systematic study of alternatively spliced genes of higher eukaryotes by generating splicing graphs for the compact visual representation of transcript diversity from a single gene. Taking a set of transcripts in General Feature Format as input, ASGS identifies distinct reference and variable exons, generates a transcript splicing graph, an exon summary, splicing events classification and a single line graph to facilitate experimental analysis. This freely available web service can be accessed at http://asgs.biolinfo.org.

Figure 1

Transcript diversity information for the human hyaluronidase gene, HYAL1 (hyaluronoglucosaminidase 1, ENSG00000114378) (a) from the ASG (7), (b) from the ASD (10) and from ASGS (c) as a splicing graph and (d) a single line splice diagram and (e) the splicing graph diagram using the DEDB formalism (8).