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. 2006 Jul 1;34(Web Server issue):W79-83.
doi: 10.1093/nar/gkl216.

ProSAT2--Protein Structure Annotation Server

R R Gabdoulline  1 , S Ulbrich, S Richter, R C Wade
Affiliations

ProSAT2--Protein Structure Annotation Server

R R Gabdoulline et al. Nucleic Acids Res. .

Abstract

ProSAT2 is a server to facilitate interactive visualization of sequence-based, residue-specific annotations mapped onto 3D protein structures. As the successor of ProSAT (Protein Structure Annotation Tool), it includes its features for visualizing SwissProt and PROSITE functional annotations. Currently, the ProSAT2 server can perform automated mapping of information on variants and mutations from the UniProt KnowledgeBase and the BRENDA enzyme information system onto protein structures. It also accepts and maps user-prepared annotations. By means of an annotation selector, the user can interactively select and group residue-based information according to criteria such as whether a mutation affects enzyme activity. The visualization of the protein structures is based on the WebMol Java molecular viewer and permits simultaneous highlighting of annotated residues and viewing of the corresponding descriptive texts. ProSAT2 is available at http://projects.villa-bosch.de/mcm/database/prosat2/.

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Figures

Figure 1
Figure 1
Screenshot showing a ProSAT2 session. At upper left is the ProSAT2 Selector window, at lower left the WebMol 3D structure visualization window. On the right hand side are the windows that pop up after requesting detailed information from the residue-highlighting panel (in the middle with coloured buttons).
Figure 2
Figure 2
Flowchart illustrating how ProSAT2 extracts residue-specific data from databases and permits the user to sort this and highlight residues on 3D protein structures along with information on their function or the impact of their mutation. The steps with user intervention are in grey. The databases used are shown in circles.
See this image and copyright information in PMC

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