doi: 10.1385/1-59745-037-5:273.
Lentiviral vector-mediated gene transfer in embryonic stem cells
Affiliations
- PMID: 16845997
- DOI: 10.1385/1-59745-037-5:273
Item in Clipboard
Lentiviral vector-mediated gene transfer in embryonic stem cells
Methods Mol Biol.
2006.
Abstract
The major limitations in gene transduction to embryonic stem (ES) cells are (1) low efficiency of gene delivery and (2) suppression of gene expression after integration into the host genome. A human immunodeficiency virus type I (HIV-1)-based lentiviral vector has been demonstrated to be an excellent tool for stable and efficient gene expression in ES cells. Here, we introduce a protocol for lentiviral vector-mediated transgene expression in murine ES cells. Using lentiviral vectors expressing LacZ, green fluorescent protein, and Cre recombinase, we demonstrate the efficiency and utility of the vectors in ES cell study.
Similar articles
-
Lentivirus-based gene delivery in mouse embryonic stem cells.Artif Organs. 2004 Mar;28(3):271-7. doi: 10.1111/j.1525-1594.2004.47297.x. Artif Organs. 2004. PMID: 15046626
-
Use of simian immunodeficiency virus vectors for simian embryonic stem cells.Methods Mol Biol. 2006;329:295-303. doi: 10.1385/1-59745-037-5:295. Methods Mol Biol. 2006. PMID: 16845999
-
Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos.Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2140-5. doi: 10.1073/pnas.251682798. Proc Natl Acad Sci U S A. 2002. PMID: 11854510 Free PMC article.
-
Lentiviral vectors and cardiovascular diseases: a genetic tool for manipulating cardiomyocyte differentiation and function.Gene Ther. 2012 Jun;19(6):642-8. doi: 10.1038/gt.2012.19. Epub 2012 Mar 1. Gene Ther. 2012. PMID: 22378345 Review.
-
Genetic manipulation of primate embryonic and hematopoietic stem cells with simian lentivirus vectors.Trends Cardiovasc Med. 2003 Apr;13(3):106-10. doi: 10.1016/s1050-1738(02)00253-0. Trends Cardiovasc Med. 2003. PMID: 12691674 Review.
Cited by
-
Immortalization of human normal and NF1 neurofibroma Schwann cells.Lab Invest. 2016 Oct;96(10):1105-15. doi: 10.1038/labinvest.2016.88. Epub 2016 Sep 12. Lab Invest. 2016. PMID: 27617404
-
Bacterial delivery of nuclear proteins into pluripotent and differentiated cells.PLoS One. 2011 Jan 27;6(1):e16465. doi: 10.1371/journal.pone.0016465. PLoS One. 2011. PMID: 21304583 Free PMC article.
-
Embryonic stem cell-derived neural precursor grafts for treatment of temporal lobe epilepsy.Neurotherapeutics. 2009 Apr;6(2):263-77. doi: 10.1016/j.nurt.2009.01.011. Neurotherapeutics. 2009. PMID: 19332319 Free PMC article. Review.
-
Emerging Applications of Nanotechnology in Healthcare Systems: Grand Challenges and Perspectives.Pharmaceuticals (Basel). 2021 Jul 21;14(8):707. doi: 10.3390/ph14080707. Pharmaceuticals (Basel). 2021. PMID: 34451803 Free PMC article. Review.
-
Imaging of STAT3 signaling pathway during mouse embryonic stem cell differentiation.Stem Cells Dev. 2009 Mar;18(2):205-14. doi: 10.1089/scd.2008.0152. Stem Cells Dev. 2009. PMID: 18576943 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
