Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis
- PMID: 16848345
Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis
Abstract
Objective: To evaluate the performance of the colorimetric resazurin microtiter assay (REMA) method for the detection of ofloxacin resistance.
Methods: A panel of 120 multidrug-resistant Mycobacterium tuberculosis strains was tested blindly by the REMA method and compared with the results obtained using the BACTEC 460 method.
Result: A very good correlation was observed between the two methods.
Conclusion: The REMA method is simple, rapid and can be an inexpensive alternative procedure for the rapid detection of anti-tuberculosis drug resistance in laboratories with limited resources.
Similar articles
-
Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays.J Clin Microbiol. 2005 Apr;43(4):1612-6. doi: 10.1128/JCM.43.4.1612-1616.2005. J Clin Microbiol. 2005. PMID: 15814974 Free PMC article.
-
Evaluation of the resazurin assay for the detection of multidrug-resistant Mycobacterium tuberculosis in Madagascar.Int J Tuberc Lung Dis. 2007 Jun;11(6):683-8. Int J Tuberc Lung Dis. 2007. PMID: 17519102
-
A new rapid and simple colorimetric method to detect pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide.J Antimicrob Chemother. 2006 Aug;58(2):327-31. doi: 10.1093/jac/dkl231. Epub 2006 Jun 2. J Antimicrob Chemother. 2006. PMID: 16751203
-
Rapid drug resistance detection in Mycobacterium tuberculosis: a review of colourimetric methods.Clin Microbiol Infect. 2007 Aug;13(8):754-62. doi: 10.1111/j.1469-0691.2007.01698.x. Epub 2007 Mar 22. Clin Microbiol Infect. 2007. PMID: 17378933 Review.
-
Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis.J Microbiol Methods. 2008 Oct;75(2):161-6. doi: 10.1016/j.mimet.2008.06.015. Epub 2008 Jun 25. J Microbiol Methods. 2008. PMID: 18627779 Review.
Cited by
-
Correlation of different phenotypic drug susceptibility testing methods for four fluoroquinolones in Mycobacterium tuberculosis.J Antimicrob Chemother. 2016 May;71(5):1233-40. doi: 10.1093/jac/dkv499. Epub 2016 Feb 6. J Antimicrob Chemother. 2016. PMID: 26851609 Free PMC article.
-
Rapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system.J Clin Microbiol. 2008 Dec;46(12):3952-6. doi: 10.1128/JCM.01171-08. Epub 2008 Oct 22. J Clin Microbiol. 2008. PMID: 18945838 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
