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. 2006 Aug;47(8):1175-81.
doi: 10.1093/pcp/pcj086. Epub 2006 Jul 18.

Purification and characterization of plant dynamin from tobacco BY-2 cells

Takahiro Hamada  1 , Hisako Igarashi, Maki Yao, Takashi Hashimoto, Teruo Shimmen, Seiji Sonobe
Affiliations

Purification and characterization of plant dynamin from tobacco BY-2 cells

Takahiro Hamada et al. Plant Cell Physiol. 2006 Aug.

Abstract

We purified an 84 kDa polypeptide from the MAP (microtubule-associated protein) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography-tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homolog of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form a filamentous structure. When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro.

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