Binding characteristics of alpha 1-adrenoceptors in bovine prostate using a radioligand binding assay
- PMID: 1686059
- DOI: 10.1248/bpb1978.14.315
Binding characteristics of alpha 1-adrenoceptors in bovine prostate using a radioligand binding assay
Abstract
Binding characteristics of alpha 1-adrenoceptors in the bovine prostate were examined using a radioligand binding assay. [3H]Bunazosin was used as a radioligand. The optimal incubation conditions for the radioligand binding assay were determined: incubation time (30 min), protein concentration (0.2 mg/tube) and temperature (30 degrees C). Scatchard plots for alpha 1-adrenoceptors in bovine prostates were linear and the Kd and Bmax values were 0.61 nM and 49.8 fmol/mg protein, respectively. The pKi value of terazosin was significantly higher than those of prazosin, bunazosin or phentolamine in the bovine prostate. Since this radioligand binding assay using [3H]bunazosin demonstrated the presence of alpha 1-adrenoceptors in bovine prostates, this method is useful for the assessment of alpha 1-blockers of new chemicals synthesized for the treatment of the benign prostatic hyperplasia.
Similar articles
-
[3H]bunazosin, a novel selective radioligand of alpha 1 adrenoceptors in human prostates.J Urol. 1991 Sep;146(3):877-80. doi: 10.1016/s0022-5347(17)37952-1. J Urol. 1991. PMID: 1714973
-
Binding characteristics of naftopidil and alpha 1-adrenoceptor antagonists to prostatic alpha-adrenoceptors in benign prostatic hypertrophy.Life Sci. 1992;50(2):127-35. doi: 10.1016/0024-3205(92)90294-y. Life Sci. 1992. PMID: 1370569
-
[Characterization and localization of alpha 1-adrenoceptors in human prostate--with special reference to the zonal difference in receptor distribution].Nihon Hinyokika Gakkai Zasshi. 1991 Aug;82(8):1241-9. doi: 10.5980/jpnjurol1989.82.1241. Nihon Hinyokika Gakkai Zasshi. 1991. PMID: 1717729 Japanese.
-
Identification and characterization of 3H-prazosin binding to alpha 1-adrenoceptors in bovine teat muscles.Arch Int Pharmacodyn Ther. 1985 Jun;275(2):189-98. Arch Int Pharmacodyn Ther. 1985. PMID: 2992402
-
Alpha-blocking potencies of antihypertensive agents (prazosin, terazosin, bunazosin, SGB-1534 and ketanserin) having with quinazoline or quinazolinedione as assessed by radioligand binding assay methods in rat brain.J Pharmacobiodyn. 1989 Mar;12(3):170-4. doi: 10.1248/bpb1978.12.170. J Pharmacobiodyn. 1989. PMID: 2569512