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. 2007 Feb;9(1):8-13.
doi: 10.1016/j.jfms.2006.05.008. Epub 2006 Jul 24.

Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

Mark D G Pinches  1 , Christopher R Helps, Tim J Gruffydd-Jones, Kathy Egan, Oswald Jarrett, Séverine Tasker
Affiliations

Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

Mark D G Pinches et al. J Feline Med Surg. 2007 Feb.

Abstract

In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.

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Figures

Fig 1
Fig 1
ROC curve RT-PCR CT – ELISA.
Fig 2
Fig 2
ROC curve RT-PCR CT – VI.
See this image and copyright information in PMC

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References

    1. Dean R., Harley R., Helps C., Caney S., Gruffydd-Jones T. Use of quantitative real-time PCR to monitor the response of Chlamydophila felis infection to doxycycline treatment, Journal of Clinical Microbiology 43 (4), 2005, 1858–1864. - PMC - PubMed
    1. Flynn J.N., Dunham S.P., Watson V., Jarrett O. Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection, Journal of Virology 76 (5), 2002, 2306–2315. - PMC - PubMed
    1. Hardy W.D., Jr., McClelland A.J., Zuckerman E.E., Hess P.W., Essex M., Cotter S.M., MacEwen E.G., Hayes A.A. Prevention of the contagious spread of feline leukaemia virus and the development of leukaemia in pet cats, Nature 263 (5575), Sep 23, 1976, 326–328. - PubMed
    1. Hofmann-Lehmann R., Huder J.B., Gruber S., Boretti F., Sigrist B., Lutz H. Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats, Journal of General Virology 82 (Pt 7), 2001, 1589–1596. - PubMed
    1. Hoover E.A., Olsen R.G., Hardy W.D., Jr., Schaller J.P., Mathes L.E., Cockerell G.L. Biologic and immunologic response of cats to experimental infection with feline leukaemia virus, Bibliotheca Haematologica 43, 1975, 180–183. - PubMed

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