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. 2007 Feb;9(1):8-13.
doi: 10.1016/j.jfms.2006.05.008. Epub 2006 Jul 24.

Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

Mark D G Pinches  1 , Christopher R Helps, Tim J Gruffydd-Jones, Kathy Egan, Oswald Jarrett, Séverine Tasker
Affiliations

Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

Mark D G Pinches et al. J Feline Med Surg. 2007 Feb.

Abstract

In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.

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Figures

Fig 1
Fig 1
ROC curve RT-PCR CT – ELISA.
Fig 2
Fig 2
ROC curve RT-PCR CT – VI.
See this image and copyright information in PMC

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