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. 2006 Jul;14(7):499-504.

[Effects of c-met-siRNA on the growth and invasion of hepatocellular carcinoma MHCC97-H cells]

[Article in Chinese]
Bin Xie  1 , De-guo Tang, Jia-hong Dong
Affiliations
  • PMID: 16867270

[Effects of c-met-siRNA on the growth and invasion of hepatocellular carcinoma MHCC97-H cells]

[Article in Chinese]
Bin Xie et al. Zhonghua Gan Zang Bing Za Zhi. 2006 Jul.

Abstract

Objective: To explore the effects of c-met-siRNA on the growth and invasion of hepatocellular carcinoma MHCC97-H cells by pSuppressorRetro/c-met-siRNA recombinant plasmid transfection.

Methods: Recombinant plasmid transfection to Phoenix A cells was constructed using the lipofectin method and then the retrovirals containing c-met-siRNA were used to infect target cells MHCC97-H. In vitro, c-met expression was tested by Western blot. Cell proliferation, motility and invasiveness were studied using MTT, cell migration assay, and cell invasion assay, respectively.

Results: The expression of c-met decreased significantly in MHCC97-H cells, and the most effective site of the target sequence was at 537. The growth, motility and invasiveness of MHCC97-H cells were inhibited.

Conclusion: The results indicate that c-met-siRNA can down-regulate the expression of c-met and inhibit hepatocellular carcinoma cell proliferation, motility and invasiveness.

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