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. 1987;5(7):649-58.
doi: 10.1016/0731-7085(87)80077-8.

On the use of fluorescent labels in immunoassay

M P Bailey  1 , B F Rocks, C Riley
Affiliations

On the use of fluorescent labels in immunoassay

M P Bailey et al. J Pharm Biomed Anal. 1987.

Abstract

The principles and use of fluorescent labels in immunoassay are reviewed. Comparison is made with radio- and chemiluminescent immunoassays. Possible fluorescent labels are listed. The following types of assay are described: separation fluoroimmunoassay, immunofluorometric assay, fluorescence enhancement and fluorescence quenching assay, fluorescence polarization immunoassay, fluorescence energy transfer immunoassay, fluorescence protection immunoassay, alternative binding immunoassay, release fluoroimmunoassay and time-resolved fluorimetry and phosphorimetry.

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