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. 1991 Sep-Oct;12(5):897-908.
doi: 10.1016/0196-9781(91)90035-n.

Identification of RFamide neuropeptides in the medicinal leech

B D Evans  1 , J Pohl, N A Kartsonis, R L Calabrese
Affiliations

Identification of RFamide neuropeptides in the medicinal leech

B D Evans et al. Peptides. 1991 Sep-Oct.

Abstract

Using a four-step reverse phase HPLC separation and RIA, five RFamide peptides were purified from CNS extracts of the leech Hirudo medicinalis. YMRFamide, FMRFamide, YLRFamide, FLRFamide, and GGKYMRFamide were identified by a combination of antiserum specificity in RIA, Edman degradation, and mass spectrometry. At least three of these five endogenous peptides can modulate neuromuscular interactions in the leech (38). FMRFamide-like immunoreactivity was selectively released from neural processes on isolated heart tubes in the presence of calcium and depolarizing levels of potassium.

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