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Comparative Study
. 2006 Aug;50(8):2860-2.
doi: 10.1128/AAC.00303-06.

Resistance levels and rpoB gene mutations among in vitro-selected rifampin-resistant Mycobacterium tuberculosis mutants

Emma Huitric  1 , Jim Werngren, Pontus Juréen, Sven Hoffner
Affiliations
Comparative Study

Resistance levels and rpoB gene mutations among in vitro-selected rifampin-resistant Mycobacterium tuberculosis mutants

Emma Huitric et al. Antimicrob Agents Chemother. 2006 Aug.

Abstract

The distribution and resistance levels of 189 in vitro-selected rifampin-resistant Mycobacterium tuberculosis mutants of Beijing and other genotypes were determined. Apart from a higher amount of codon 522 point mutations and large deletions, a spread of mutations similar to that reported for clinical isolates was seen. Most mutations were correlated with high-level resistance; a lower level, or a MIC of <16 mg/liter, was associated with codon 522 mutations. Multiple mutations were detected in two Beijing mutants.

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Figures

FIG. 1.
FIG. 1.
Distribution of in vitro-selected, rpoB mutation sites in 89 Beijing and 99 non-Beijing genotype M. tuberculosis mutants. The mutant lacking an RRDR mutation is not included. tot, total; SNP's, single nucleotide polymorphism.
See this image and copyright information in PMC

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