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. 2006 Aug 2;26(31):8017-20.
doi: 10.1523/JNEUROSCI.2728-06.2006.

Antibodies as valuable neuroscience research tools versus reagents of mass distraction

Kenneth J Rhodes  1 , James S Trimmer
Affiliations

Antibodies as valuable neuroscience research tools versus reagents of mass distraction

Kenneth J Rhodes et al. J Neurosci. .
No abstract available

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Figures

Figure 1.
Figure 1.
Examples of nonspecific and specific nickel-enhanced diaminobenzidine staining for somatodendritic ion channels from adult rat neocortex. A, A common nonspecific staining pattern. In this case, an ELISA-positive mAb against Kv3.1 yields cytoplasmic (not plasma membrane) staining in neocortical layer V pyramidal neurons, which do not express Kv3.1 mRNA (Weiser et al., 1994). B, Specific staining for Kv2.1. Note the crisp plasma membrane-associated staining, as expected for an ion channel, and little cytoplasmic or nuclear signal. Insets are higher-magnification views of the boxed area.
Figure 2.
Figure 2.
Validation of antibody staining for a dendritic ion channel using knock-out mice. Pattern of double immunofluorescence staining of an anti-Kv4.2 mAb (K57/1; red) and an anti-Kv2.1 pAb (green) against hippocampi from adult wild-type (A) and Kv4.2 knock-out (B) mice.
See this image and copyright information in PMC

References

    1. Bekele-Arcuri Z, Matos MF, Manganas L, Strassle BW, Monaghan MM, Rhodes KJ, Trimmer JS (1996). Generation and characterization of subtype-specific monoclonal antibodies to K+ channel α- and β-subunit polypeptides. Neuropharmacology 35:851–865. - PubMed
    1. Rhodes KJ, Monaghan MM, Barrezueta NX, Nawoschik S, Bekele-Arcuri Z, Matos MF, Nakahira K, Schechter LE, Trimmer JS (1996). Voltage-gated K+ channel β subunits: expression and distribution of Kvβ1 and Kvβ2 in adult rat brain. J Neurosci 16:4846–4860. - PMC - PubMed
    1. Saper CB, Sawchenko PE (2003). Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry. J Comp Neurol 465:161–163. - PubMed
    1. Weiser M, Vega-Saenz de Miera E, Kentros C, Moreno H, Franzen L, Hillman D, Baker H, Rudy B (1994). Differential expression of Shaw-related K+ channels in the rat CNS. J Neurosci 14:949–972. - PMC - PubMed

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