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. 2006 Aug;72(8):5673-6.
doi: 10.1128/AEM.01894-05.

Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus INA288

Takeshi Ito  1 , Tomonori Ikeya, Ken Sahara, Hisanori Bando, Shin-ichiro Asano
Affiliations

Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus INA288

Takeshi Ito et al. Appl Environ Microbiol. 2006 Aug.

Abstract

Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.

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Figures

FIG. 1.
FIG. 1.
Organization of the genes in the cry30Ba and cry44Aa operons. The positions and directions of the genes are indicated by open arrows. The eight regions conserved among Cry proteins are indicated by black rectangles. The arrowhead and asterisks indicate the putative promoter region and the transcriptional terminator, respectively. Black diamonds indicate tentative ribosome binding sites.
FIG. 2.
FIG. 2.
Scanning electron microscopy showing spores and crystalline inclusions produced by the B. thuringiensis parental strain and transformants. Magnification, ×10,000.
FIG. 3.
FIG. 3.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of crystal proteins produced by the B. thuringiensis parental strain and transformants. Lane 1, molecular mass markers.
See this image and copyright information in PMC

References

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