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. 2006 Aug;44(8):3025-7.
doi: 10.1128/JCM.00068-06.

Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples

F Franco-Alvarez de Luna  1 , P Ruiz, J Gutiérrez, M Casal
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Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples

F Franco-Alvarez de Luna et al. J Clin Microbiol. 2006 Aug.

Abstract

We evaluated GenoType Mycobacteria Direct (GTMD), a novel commercial assay based on nucleic acid sequence-based amplification technology, for the detection of Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, and M. malmoense directly from clinical specimens. A total of 134 respiratory and extrapulmonary samples from 65 patients were processed. Sensitivity, specificity, positive predictive, and negative predictive values for GTMD were 92, 100, 100, and 77%, respectively. The GMTD technique is useful, reliable, and rapid when used during the normal routine of a clinical laboratory.

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References

    1. American Thoracic Society. 2000. Diagnostic standards and classification of tuberculosis in adults and children. Am. J. Respir. Crit. Care Med. 161:1376-1395. - PubMed
    1. Boden, D., M. Weizenegger, K. Benz, W. Ponstingl, M. Hengstler, S. Ruschgerdes, A. Fahr, and J. Sartel. 1998. Reverse hybridization assay for rapid identification of Mycobacteria from culture samples. Clin. Lab. 44:687-692.
    1. Bodmer, T., A. Gurtner, M. Scholkmann, and L. Matter. 1997. Evaluation of the COBAS AMPLICOR MTB system. J. Clin. Microbiol. 35:1604-1605. - PMC - PubMed
    1. Centers for Disease Control and Prevention. 2000. Nucleic acid amplification tests for tuberculosis. Morb. Mortal. Wkly. Rep. 49:593-594. - PubMed
    1. Chan, A. B., and J. D. Fox. 1999. NASBA and other transcription-based amplification methods for research and diagnostic microbiology. Rev. Med. Microbiol. 10:185-196.

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