Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Aug 26;397(2):510-8.
doi: 10.1016/0005-2744(75)90141-2.

A physical explanation of the EPR spectrum observed during catalysis by enzymes utilizing coenzyme B12

K L Schepler, W R Dunham, R H Sands, J A Fee, R H Abeles
Free article

A physical explanation of the EPR spectrum observed during catalysis by enzymes utilizing coenzyme B12

K L Schepler et al. Biochim Biophys Acta. .
Free article

Abstract

We have proposed that the "doublet" EPR spectra observed during catalysis by a number of coenzyme B12-requiring enzymes arises from a weak electrostatic exchange interaction between an organic free radical and low spin Co(II), B12r. By varying the magnitude of the exchange of coupling we have quite accurately simulated the published EPR spectra from the enzyme systems: diol dehydrase, glycerol dehydrase, ribonucleotide reductase, and ethanolamine ammon-ia lyase. A dipolar model was shown to be incompatible with the observed properties of these systems.

PubMed Disclaimer

Publication types