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Comment
. 2006 Aug 15;103(33):12213-4.
doi: 10.1073/pnas.0605344103. Epub 2006 Aug 7.

Embryonic stem cells make human T cells

Heather E Fleming  1 , David T Scadden
Affiliations
Comment

Embryonic stem cells make human T cells

Heather E Fleming et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

Conflict of interest statement: No conflicts declared.

Figures

Fig. 1.
Fig. 1.
Generation of blood cells from ES cells. (Left) mESC were grown on OP9 feeders followed by Dll-1+ OP9 cells and subsequent transfer to fetal thymic organ culture (FTOC). Transplant of the seeded FTOC into RAG−/− hosts generated T cells responsive to an in vivo viral challenge (12). (Center) hESC were differentiated into EBs for 10 days. CD45−, PECAM-1+, Flk-1+, and VE-cadherin+ (PFV) cells were isolated and grown in serum-free growth conditions and then injected intrafemorally to prevent aggregation caused by murine serum. Localized and minimal contralateral multilineage but no T lineage engraftment was obtained (8). (Right) hESC were cultured on OP9 stroma for 10–14 days, and the resulting CD34+ or CD133+ cells were isolated. SCID or RAG−/− mice harboring a human fetal thymic/liver rudiment under the renal capsule were irradiated, and ES cell-derived hematopoietic progenitors were injected within the thymus/liver (thy/liv) graft. Biopsies from the graft contained mature T cells that could be activated by anti-CD3 in vitro (1).
See this image and copyright information in PMC

Comment on

  • T lineage differentiation from human embryonic stem cells.
    Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA. Galic Z, et al. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11742-7. doi: 10.1073/pnas.0604244103. Epub 2006 Jul 14. Proc Natl Acad Sci U S A. 2006. PMID: 16844782 Free PMC article.

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