Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Feb 1;265(3):789-98.
doi: 10.1042/bj2650789.

Cloning and sequencing of protochlorophyllide reductase

P M Darrah  1 , S A Kay, G R Teakle, W T Griffiths
Affiliations

Cloning and sequencing of protochlorophyllide reductase

P M Darrah et al. Biochem J. .

Abstract

Putative protochlorophyllide reductase cDNA clones (252 and 113) were isolated from an etiolated-oat (Avena sativa) cDNA library. These were used to indirectly characterize a further clone, p127, isolated from a lambda-phage gt11 cDNA library. The latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a CNBr-cleavage fragment of the purified reductase, p127 codes for more than 95% of the reductase protein.

PubMed Disclaimer

References

    1. Eur J Biochem. 1976 AUG 1;67(1):247-56 - PubMed
    1. Nature. 1976 Mar 25;260(5549):359-60 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3196-200 - PubMed

Associated data